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human testis marathon ready cdna library  (TaKaRa)


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    TaKaRa human testis marathon ready cdna library
    Human Testis Marathon Ready Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+testis+marathon+ready+cdna+library/us07893201-647-15-20
    Average 86 stars, based on 1 article reviews
    human testis marathon ready cdna library - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis
    Article Snippet: In addition, the expression of the SPATA3 was predicted by The Human Protein Atlas (proteinatlas.org/) and Uniprot database ( https://www.uniprot.org/ ). .. In our previous study, the full-length cDNA of SPATA3 was amplified using Human Testis Marathon-Ready cDNA library (Clontech (USA) as the template, and the full-length gene sequences of the open reading frames of the human open reading frame SPATA3 isoforms were obtained via cloning with the pGM-T vector ( ). ..

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Arp7A (GenBank TM : NM_006687 ) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into the NotI-EcoRI sites of CB6-N-GFP ( ) to generate GFP-Arp7A for expression in mammalian cells. ..

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis.
    Article Snippet: The domains and other features of the SPaTa3 isoforms were predicted using the online interPro database (hwww.ebi.ac.uk/interpro/search/sequence/). in addition, the expression of the SPaTa3 was predicted by The Human Protein atlas (proteinatlas.org/) and uniprot database (https://www.uniprot.org/). .. Molecular cloning for AS variants of SPATA3 using the pcDNA5 and pEGFP‐C3 vectors. in our previous study, the full‐length cdna of SPATA3 was amplified using Human Testis Marathon‐ready cdna library (clontech (uSa) as the template, and the full‐length gene sequences of the open reading frames of the human open reading frame SPaTa3 isoforms were obtained via cloning with the pGM‐T vector (28). using this plasmid as a template, amplify the full‐length open reading frame sequences of SPaTa3 isoforms via Pcr. .. The expres‐ sion vector pcdna5 was double digested with EcorV and BamHi restriction enzymes, the expression vector peGFP‐c3 was double digested with Ecori and BamHi restriction enzymes, and the SPaTa3 gene fragment was digested using the same restriction enzymes as the respective vectors and ligated into the digested backbones. novel isoforms were cloned and inserted into the pcdna5 plasmid (clontech; Takara Bio uSa, inc.) to generate recombinant plasmids, which included pcdna5‐SPATA3‐i1, pcdna5‐SPATA3‐i2, pcdna5‐SPATA3‐i3 and pcdna5‐SPATA3‐i4.

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Mammalian Expression Vectors, Pulldown, and Immunoprecipitation Assays—Arp7A (GenBankTM: NM_006687) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into theNotI-EcoRI sites of CB6N-GFP (30) to generate GFP-Arp7A for expression inmammalian cells. ..

    cDNA Library Assay:

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis
    Article Snippet: In addition, the expression of the SPATA3 was predicted by The Human Protein Atlas (proteinatlas.org/) and Uniprot database ( https://www.uniprot.org/ ). .. In our previous study, the full-length cDNA of SPATA3 was amplified using Human Testis Marathon-Ready cDNA library (Clontech (USA) as the template, and the full-length gene sequences of the open reading frames of the human open reading frame SPATA3 isoforms were obtained via cloning with the pGM-T vector ( ). ..

    Article Title: Ankyrin facilitates intracellular trafficking of ? 1 -Na + -K + -ATPase in polarized cells
    Article Snippet: This fragment was ligated into pEGFP-N1 (Clontech) that had been previously digested with Bam HI, treated with Mung Bean Nuclease, buffer exchanged, and digested again with Bgl II. .. A similar strategy was used to make cytoplasmic domain-deleted tso45-VSV-G-pEGFP ( clone 330 ) but using antisense oligonucleotide 55633 (5′-CGGAATTCATAAATACCAACTCGGAG-3′), which deleted the last 24 amino acids of VSV-G. tso45-VSV-MAB-pEGFP ( clone 337 ) was assembled by amplifying MAB from a human testis marathon ready cDNA library (Clontech) using sense oligonucleotide 55628 (5′-CGGAATTCTCCTACTATCAAGAAGCTAA-3′) and antisense oligonucleotide 29889 (5′-CGGAATTCTTAATGTTCAATTTCTGCAGCGATGG-3′), both with 5′ Eco RI sites. ..

    Article Title: Characterization and molecular cloning of novel isoforms of human spermatogenesis associated gene SPATA3.
    Article Snippet: This study aimed to clone and characterize novel isoforms of the human SPATA3 gene.. The isoforms of SPATA3 gene was cloned into pGMT vector using human testis cDNA as template, and Sanger sequencing was performed.. Their characterizations and tissue-specific expression profiles were analyzed.

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Arp7A (GenBank TM : NM_006687 ) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into the NotI-EcoRI sites of CB6-N-GFP ( ) to generate GFP-Arp7A for expression in mammalian cells. ..

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis.
    Article Snippet: The domains and other features of the SPaTa3 isoforms were predicted using the online interPro database (hwww.ebi.ac.uk/interpro/search/sequence/). in addition, the expression of the SPaTa3 was predicted by The Human Protein atlas (proteinatlas.org/) and uniprot database (https://www.uniprot.org/). .. Molecular cloning for AS variants of SPATA3 using the pcDNA5 and pEGFP‐C3 vectors. in our previous study, the full‐length cdna of SPATA3 was amplified using Human Testis Marathon‐ready cdna library (clontech (uSa) as the template, and the full‐length gene sequences of the open reading frames of the human open reading frame SPaTa3 isoforms were obtained via cloning with the pGM‐T vector (28). using this plasmid as a template, amplify the full‐length open reading frame sequences of SPaTa3 isoforms via Pcr. .. The expres‐ sion vector pcdna5 was double digested with EcorV and BamHi restriction enzymes, the expression vector peGFP‐c3 was double digested with Ecori and BamHi restriction enzymes, and the SPaTa3 gene fragment was digested using the same restriction enzymes as the respective vectors and ligated into the digested backbones. novel isoforms were cloned and inserted into the pcdna5 plasmid (clontech; Takara Bio uSa, inc.) to generate recombinant plasmids, which included pcdna5‐SPATA3‐i1, pcdna5‐SPATA3‐i2, pcdna5‐SPATA3‐i3 and pcdna5‐SPATA3‐i4.

    Article Title: Hemopoietin receptor protein, NR12
    Article Snippet: .. Under the same PCR condition to those described in (4) for 5′-RACE method except using Human Testis Marathon-Ready cDNA Library (Clontech#7414-1) as the template. ..

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Mammalian Expression Vectors, Pulldown, and Immunoprecipitation Assays—Arp7A (GenBankTM: NM_006687) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into theNotI-EcoRI sites of CB6N-GFP (30) to generate GFP-Arp7A for expression inmammalian cells. ..

    Article Title: Hemopoietin receptor protein, NR12
    Article Snippet: .. Under the same PCR condition to those described in (4) for 5′-RACE method except using Human Testis Marathon-Ready cDNA Library (Clontech#7414-1) as the template. ..

    Cloning:

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis
    Article Snippet: In addition, the expression of the SPATA3 was predicted by The Human Protein Atlas (proteinatlas.org/) and Uniprot database ( https://www.uniprot.org/ ). .. In our previous study, the full-length cDNA of SPATA3 was amplified using Human Testis Marathon-Ready cDNA library (Clontech (USA) as the template, and the full-length gene sequences of the open reading frames of the human open reading frame SPATA3 isoforms were obtained via cloning with the pGM-T vector ( ). ..

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis.
    Article Snippet: The domains and other features of the SPaTa3 isoforms were predicted using the online interPro database (hwww.ebi.ac.uk/interpro/search/sequence/). in addition, the expression of the SPaTa3 was predicted by The Human Protein atlas (proteinatlas.org/) and uniprot database (https://www.uniprot.org/). .. Molecular cloning for AS variants of SPATA3 using the pcDNA5 and pEGFP‐C3 vectors. in our previous study, the full‐length cdna of SPATA3 was amplified using Human Testis Marathon‐ready cdna library (clontech (uSa) as the template, and the full‐length gene sequences of the open reading frames of the human open reading frame SPaTa3 isoforms were obtained via cloning with the pGM‐T vector (28). using this plasmid as a template, amplify the full‐length open reading frame sequences of SPaTa3 isoforms via Pcr. .. The expres‐ sion vector pcdna5 was double digested with EcorV and BamHi restriction enzymes, the expression vector peGFP‐c3 was double digested with Ecori and BamHi restriction enzymes, and the SPaTa3 gene fragment was digested using the same restriction enzymes as the respective vectors and ligated into the digested backbones. novel isoforms were cloned and inserted into the pcdna5 plasmid (clontech; Takara Bio uSa, inc.) to generate recombinant plasmids, which included pcdna5‐SPATA3‐i1, pcdna5‐SPATA3‐i2, pcdna5‐SPATA3‐i3 and pcdna5‐SPATA3‐i4.

    Plasmid Preparation:

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis
    Article Snippet: In addition, the expression of the SPATA3 was predicted by The Human Protein Atlas (proteinatlas.org/) and Uniprot database ( https://www.uniprot.org/ ). .. In our previous study, the full-length cDNA of SPATA3 was amplified using Human Testis Marathon-Ready cDNA library (Clontech (USA) as the template, and the full-length gene sequences of the open reading frames of the human open reading frame SPATA3 isoforms were obtained via cloning with the pGM-T vector ( ). ..

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis.
    Article Snippet: The domains and other features of the SPaTa3 isoforms were predicted using the online interPro database (hwww.ebi.ac.uk/interpro/search/sequence/). in addition, the expression of the SPaTa3 was predicted by The Human Protein atlas (proteinatlas.org/) and uniprot database (https://www.uniprot.org/). .. Molecular cloning for AS variants of SPATA3 using the pcDNA5 and pEGFP‐C3 vectors. in our previous study, the full‐length cdna of SPATA3 was amplified using Human Testis Marathon‐ready cdna library (clontech (uSa) as the template, and the full‐length gene sequences of the open reading frames of the human open reading frame SPaTa3 isoforms were obtained via cloning with the pGM‐T vector (28). using this plasmid as a template, amplify the full‐length open reading frame sequences of SPaTa3 isoforms via Pcr. .. The expres‐ sion vector pcdna5 was double digested with EcorV and BamHi restriction enzymes, the expression vector peGFP‐c3 was double digested with Ecori and BamHi restriction enzymes, and the SPaTa3 gene fragment was digested using the same restriction enzymes as the respective vectors and ligated into the digested backbones. novel isoforms were cloned and inserted into the pcdna5 plasmid (clontech; Takara Bio uSa, inc.) to generate recombinant plasmids, which included pcdna5‐SPATA3‐i1, pcdna5‐SPATA3‐i2, pcdna5‐SPATA3‐i3 and pcdna5‐SPATA3‐i4.

    Polymerase Chain Reaction:

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Arp7A (GenBank TM : NM_006687 ) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into the NotI-EcoRI sites of CB6-N-GFP ( ) to generate GFP-Arp7A for expression in mammalian cells. ..

    Article Title: Hemopoietin receptor protein, NR12
    Article Snippet: .. Under the same PCR condition to those described in (4) for 5′-RACE method except using Human Testis Marathon-Ready cDNA Library (Clontech#7414-1) as the template. ..

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Mammalian Expression Vectors, Pulldown, and Immunoprecipitation Assays—Arp7A (GenBankTM: NM_006687) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into theNotI-EcoRI sites of CB6N-GFP (30) to generate GFP-Arp7A for expression inmammalian cells. ..

    Article Title: Hemopoietin receptor protein, NR12
    Article Snippet: .. Under the same PCR condition to those described in (4) for 5′-RACE method except using Human Testis Marathon-Ready cDNA Library (Clontech#7414-1) as the template. ..

    Clone Assay:

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Arp7A (GenBank TM : NM_006687 ) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into the NotI-EcoRI sites of CB6-N-GFP ( ) to generate GFP-Arp7A for expression in mammalian cells. ..

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Mammalian Expression Vectors, Pulldown, and Immunoprecipitation Assays—Arp7A (GenBankTM: NM_006687) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into theNotI-EcoRI sites of CB6N-GFP (30) to generate GFP-Arp7A for expression inmammalian cells. ..

    Expressing:

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Arp7A (GenBank TM : NM_006687 ) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into the NotI-EcoRI sites of CB6-N-GFP ( ) to generate GFP-Arp7A for expression in mammalian cells. ..

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Mammalian Expression Vectors, Pulldown, and Immunoprecipitation Assays—Arp7A (GenBankTM: NM_006687) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into theNotI-EcoRI sites of CB6N-GFP (30) to generate GFP-Arp7A for expression inmammalian cells. ..

    Molecular Cloning:

    Article Title: Roles of human SPATA3 in cell proliferation and expression pattern of Spata3 in mouse testis.
    Article Snippet: The domains and other features of the SPaTa3 isoforms were predicted using the online interPro database (hwww.ebi.ac.uk/interpro/search/sequence/). in addition, the expression of the SPaTa3 was predicted by The Human Protein atlas (proteinatlas.org/) and uniprot database (https://www.uniprot.org/). .. Molecular cloning for AS variants of SPATA3 using the pcDNA5 and pEGFP‐C3 vectors. in our previous study, the full‐length cdna of SPATA3 was amplified using Human Testis Marathon‐ready cdna library (clontech (uSa) as the template, and the full‐length gene sequences of the open reading frames of the human open reading frame SPaTa3 isoforms were obtained via cloning with the pGM‐T vector (28). using this plasmid as a template, amplify the full‐length open reading frame sequences of SPaTa3 isoforms via Pcr. .. The expres‐ sion vector pcdna5 was double digested with EcorV and BamHi restriction enzymes, the expression vector peGFP‐c3 was double digested with Ecori and BamHi restriction enzymes, and the SPaTa3 gene fragment was digested using the same restriction enzymes as the respective vectors and ligated into the digested backbones. novel isoforms were cloned and inserted into the pcdna5 plasmid (clontech; Takara Bio uSa, inc.) to generate recombinant plasmids, which included pcdna5‐SPATA3‐i1, pcdna5‐SPATA3‐i2, pcdna5‐SPATA3‐i3 and pcdna5‐SPATA3‐i4.

    Immunoprecipitation:

    Article Title: Molecular Recognition of the Tes LIM2–3 Domains by the Actin-related Protein Arp7A
    Article Snippet: .. Mammalian Expression Vectors, Pulldown, and Immunoprecipitation Assays—Arp7A (GenBankTM: NM_006687) was amplified by PCR from a human testis Marathon-Ready cDNA library (Clontech) and cloned into theNotI-EcoRI sites of CB6N-GFP (30) to generate GFP-Arp7A for expression inmammalian cells. ..



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